Effect of TCF21 on A549 human lung cancer cells in nude mice
10.3969/j.issn.1006-5725.2016.08.008
- VernacularTitle:TCF21基因对人肺癌A549细胞裸鼠成瘤的影响
- Author:
Xiao LU
;
Lei XIAN
- Publication Type:Journal Article
- Keywords:
Lung neolasm;
TCF21;
A549;
Nude mice
- From:
The Journal of Practical Medicine
2016;32(8):1226-1229
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of TCF21 on human non-small cell lung cancer A549 cells , and to reveal the role of TCF21 in the development of NSCLC. Methods Overexpression of TCF21 in NSCLC A549 cells was mediated by lentivirus vector. TCF21 protein expression was identified by Western blotting assay. The experimental group, negative control group and the blank control group were set up. A549 cells were subcuta-neously seeded in BALB/c nude mice on the left armpit. Results TCF21 was successful overexpressed in the ex-perimental group. Compared with the negative control group and the blank control group , the tumor growth was slow, and the final tumor volume was significantly reduced in the experimental group. Conclusion Overexpression of TCF21 can inhibit the tumor growth of NSCLC in nude mice , indicating that TCF21 may play an important role in NSCLC development.