Impact of sperms on fertilization rate after in-vitro fertilization and embryo transfer
10.3969/j.issn.1006-5725.2016.08.021
- VernacularTitle:精子因素对体外受精－胚胎移植术中受精率的影响
- Author:
Lan MA
;
Yang LIU
;
Yin TAN
;
Jingsi CHEN
;
Xiaoling YANG
- Publication Type:Journal Article
- Keywords:
IVF-ET;
Fertilization failure;
gACE
- From:
The Journal of Practical Medicine
2016;32(8):1271-1274
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the related factors of fertilization failure after in-vitro fertilization and embryo transfer. Methods 150 patients were divided into total fertilization failure (TFF) group, low fertilization (LFR) group, and control (NFR) group according to fertilization rate. Semen was collected from the male pa-tients; the number, concentration, shape, and progressive motility of sperms were measured. Level of gACE was detected by Western blot. Logistic regression was used to explore the factors affecting fertilization rate. Results The fertilization rate and the concentration , progressive motility , and shape of sperms in were lower TFF group than in LFR group and NFR group (P < 0.05). Western blot proved that level of gACE group was higher in TFF than in LFR group and NFR group (P < 0.05). Logistic regression showed that the fertilization rate, the concen-tration , progressive motility , shape of sperms , and the level of gACE were all the independent risk factors for fertilization failure. Conclusions The concentration, progressive motility, and shape of sperms have impact on IVF. A lower expression of gACE in patients with lower fertilization rate can be used as a potential biomarker for predicting fertilization failure.
