Establishment and comparison of obese asthma models in different strains of mice
10.3969/j.issn.1001-1978.2016.02.027
- VernacularTitle:不同品系小鼠肥胖哮喘模型建立及比较
- Author:
Yiping CHEN
;
Xiaohong JIANG
;
Guangyi FENG
;
Qixiang SUN
;
Mingjie LUO
;
Chaoqian LI
- Publication Type:Journal Article
- Keywords:
obesity;
asthma;
model;
KM mice;
C57BL/6J mice;
BALB/c mice
- From:
Chinese Pharmacological Bulletin
2016;(2):288-292
- CountryChina
- Language:Chinese
-
Abstract:
Aim To establish and compare asthma models among different strains of obese mice. Methods Different strains of SPF female mice, namely Kunming ( KM ) , C57BL/6J and BALB/c mice, were randomly divided into four groups ( control group, asthma group, obesity group and obese asthma group). The mice were fed a high-fat diet or a normal diet for 12 weeks, following which they were sensitized and challenged with ovalbu-min ( OVA) or phosphate-buffered saline ( PBS) . Body weight, fat weight, liver weight, Lee′s index, OVA-specific IgE concen-tration in bronchoalveolar lavage fluid ( BALF) , serum total cho-lesterol ( TC) and triglyceride ( TG) levels, and lung and adi-pose morphologies were evaluated. The specific airway resistance ( sRaw) was measured using double-chamber plethysmography. Results The mice on a high-fat diet showed a more rapid in-crease in body weight compared with those on a normal diet. Af-ter 12 weeks of feeding, body, fat, and liver weights and Lee′s index were higher for the obese mice than for the lean mice. The adipocyte cross-sectional area was significantly greater in the obese BALB/c and KM mice than in their lean counterparts;the C57BL/6J groups showed no significant differences. The BALB/c mice demonstrated more significant symptoms of acute asthma, local inflammation and airway hyper-responsiveness ( AHR ) . Conclusion Compared with C57BL/6J and KM mice, BALB/c mice fed a high-fat diet and sensitized and challenged with OVA provide the most suitable model for evaluating the relationship between obesity and asthma.
