Different influence of simvastatin on p27 protein expressions of vascular smooth muscle cells and endo-thelial cells in rats
10.3969/j.issn.1008-0074.2016.01.14
- VernacularTitle:辛伐他汀对大鼠血管平滑肌细胞和内皮细胞 p27蛋白表达的影响
- Author:
Yanxia LIU
;
Po ZHANG
;
Xianyang ZHU
;
Lan HUANG
- Publication Type:Journal Article
- Keywords:
Myocytes,smooth muscle;
Endothelial cells;
Cyclin-dependent kinase inhibitor p27;
Simvastatin
- From:
Chinese Journal of cardiovascular Rehabilitation Medicine
2016;25(1):48-51
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe influence of simvastatin on p 27 protein (cyclin‐dependent kinase inhibitor ) expres‐sions of vascular smooth muscle cells (SMC) and endothelial cells (EC) in rats for screening new generation coating drugs of eluting stents .Methods :Primary aortic SMC and EC of rat were isolated and cultured by methods of adher‐ent and enzymatic digestion respectively .Which were inoculated on fibronectin -coated culture plates .α smooth muscle actin immunofluorescence staining was used to identify SMC ,and von Willebrand factor (vWF) immunofluo‐rescence staining was used to identify EC .SMC and EC were cultured for 24h with different concentrations of simv‐astatin (0.01 ,0.1 ,1 and 10 μmol/L) ,then Western blot was used to measure p27 protein expression .Results:Compared with blank control group ,0.01μmol/L simvastatin had no significant influence on p 27 protein expression of SMC ,but 0.1 ,1 and 10 μmol/L simvastatin significantly raised p27 protein expression of SMC [ (0.53 ± 0.08) vs .(0.86 ± 0.05) ,(1.20 ± 0.05) ,(1.60 ± 0.04)] , P< 0.01 all .Besides ,different concentrations of simvastatin had no significant influence on p27 protein expression of EC , P> 0.05 ,indicating that simvastatin only dose‐de‐pendently promoted p27 protein expression of SMC .Conclusion:Simvastatin dose -dependently promotes p27 pro‐tein expression of vascular smooth muscle cells without affecting p 27 protein expression of endothelial cells .So local application of simvastatin may inhibit restenosis and promote reendothelialization of injured vessels .
