Effect of 2-deoxy-D-glucoseon on expression of CCR5 and killing function of humanγδT cells in vitro

VernacularTitle:2－脱氧葡萄糖对体外培养人γδT细胞CCR5表达及杀伤功能影响
Author: Lu ZHENG ; Yongqiang CHEN ; Junquan LIU ; Xiaoting Lü ; Juan ZHANG ; Leiqing SUN ; Jing XU ; Zhonghai ZHOU ; Fuxing CHEN
Publication Type:Journal Article
Keywords: 2-DG; γδTcells; CCR5; Killingfunction
From: Chinese Journal of Immunology 2016;(1):29-32
CountryChina
Language:Chinese
Abstract: Objective:To investigate the effect of 2-deoxy-D-glucose (2-DG) on hunmanγδT cells on the expression of CCR5 and killing function in vitro.Methods:UsingγδT medium to cultivate peripheral blood mononuclear cell( PBMCs) in vitro.After co-cultured with various concentrations of 2-DG for 48 h,the expression of CCR5 and killing activities of γδT cells for each group were detected by flow cytometry and CCK-8 methods.Results: 2-DG could not promote the growth of γδT cells with the increase in concentration from 0 μmol/L to 1.0 μmol/L and decreased thereafter.The certain concentration ( 0-2.0 μmol/L ) of 2-DG could upregulate the expression of CCR5 in dose dependent manner.Besides,at 0.5μmol/L and 1.0μmol/L of 2-DG could increase the ex-pression of CD107a and perforin and have no effect on the granzyme B.Conclusion: Human γδT cells isolated from peripheral blood treated with 2-DG could promote the expression of CCR5 and increase the killing activities at certain concentration in vitro.