Effects of allogeneic compact bone derived-mesenchymal stem cell on proliferation,differentiation and chemokine receptor expression of T cells
10.3969/j.issn.1000-484X.2016.01.008
- VernacularTitle:骨密质来源间充质干细胞对小鼠T细胞增殖、分化及其表面趋化因子受体表达的影响
- Author:
Shukai QIAO
;
Xiaonan GUO
;
Jinhai REN
;
Xiaoling GUO
;
Yujie GUO
;
Shengxin CAI
- Publication Type:Journal Article
- Keywords:
Mesenchymal stem cells;
T cells;
Chemokine receptor;
Apoptosis
- From:
Chinese Journal of Immunology
2016;(1):37-41
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effects of allogeneic compact bone derived-mesenchymal stem cells ( CB-MSCs) on pro-liferation and differentiation of T cells,and investigate the molecular mechanisms of the immunosuppressive ability.Methods:With an established co-culture system of CB-MSCs and mouse spleen lymphocytes ( SP) in vitro,we observed the effects of CB-MSCs on prolif-eration,apoptosis and cell cycle of SP by MTS/PES assay and flow cytometry.Also,we measured the effects of CB-MSCs on regulatory T cells ( Treg) ratio and expressions of CCR5,CCR7 and CXCR3 in SP.Results:CB-MSCs could obviously inhibit the PHA-stimulated SP proliferation with a dose-dependent manner;MSCs could significantly inhibit the spontaneous apoptosis of SP and induce SP cell cycle G0/G1 phase arrest.After co-culture with SP,CB-MSCs could obviously increase the proportion of Treg in SP,down-regulate the expression of CXCR3 and CCR5,as well as up-regulate the expression of CCR7.Conclusion: Allogeneic CB-MSCs can significantly inhibit cell proliferation of SP,the mechanisms mainly involved the G0/G1 cell cycle arrest rather than apoptosis induction.In addition, CB-MSCs can exert immunomodulatory effects by increasing the Treg ratio,regulating the expressions of chemokine receptors.
