Normal and lung qi deficiency rats proteomics of lung tissue variance analysis
10.3969/j.issn.1000-484X.2016.01.010
- VernacularTitle:正常及肺气虚大鼠肺组织蛋白组学差异分析
- Author:
Ping WANG
;
Fangfang LIU
;
Junlian LI
;
Yanping MA
;
Xiaowei ZHANG
;
Yanyan LI
;
Weijun ZHANG
;
Gongding TAO
- Publication Type:Journal Article
- Keywords:
Lung qi deficiency;
Lung tissue;
Proteomics;
Variance analysis
- From:
Chinese Journal of Immunology
2016;(1):46-50
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Normal and lung qi deficiency rats of proteomics of lung tissue of rats differentially expressed and mass spectrometry.Methods:A total of 20 SD rats were randomly divided into control group,lung qi deficiency group,with 10 rats in each roup.Lung qi deficiency group model replication using composite method by intratracheal injection of lipopolysaccaride and smoked.All rats in each group before anesthesia 12 hours of fasting,water deprivation,weight after 28 days modeling,Thoracotomy to remove left lung tissue after anesthesia was 10 mg from each rat, 3 rats from each group, using two-dimensional electrophorsis analysis, two-dimensional gel electrophoresis image, peptide mass fingerprinting and electrospray tandem by mass spectrometry to protein identification.Results:2 differntial protin profiles for proteomics analysis,identified 14 protein in rat lung tissue,protein 5 expression, regulation 9 expression.Conclusion:By proteomic analysis,identify the protein have correlation with lung deficiency syndrome.
