Resveratrol ameliorates cerebral ischemia/reperfusion injury by inhibiting endo-plasmic reticulum stress in rats
10.3969/j.issn.1000-484X.2016.01.020
- VernacularTitle:白藜芦醇通过抑制内质网应激减轻大鼠脑缺血／再灌注损伤
- Author:
Xuan GAO
;
Lizhi CHEN
;
Zhenhong LIU
;
Yanhua Lü
;
Liwei XIA
;
Yongqiu LI
- Publication Type:Journal Article
- Keywords:
Resveratrol;
Cerebral ischemia;
Reperfusion injury;
Endoplasmic reticulum;
Stress
- From:
Chinese Journal of Immunology
2016;(1):92-96
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of resveratrol ( Res ) on endoplasmic reticulum stress induced by cerebral ischemia/reperfusion(I/R)injury in rats.Methods:The seventy-two male SD rats were divided randomly into three groups(n=20):sham operation group ( group S ) , I/R group and Res-treatment group ( group R ) .Focal cerebral I/R model was induced by electrocoagulation of left middle cerebral artery and occlusion of bilateral common carotid arteries followed by reperfusion after 30 min.The rats in Res group were treated with Res(50 mg/kg)i.p.7 d before the operation,once a day for 7 d.Neurological deficits were assessed at 24 h post-injury,followed by collecting the brain tissues.Cerebral infarct size was detected by TTC staining,and the water content of brain tissue were measured by wet-dry weight method.The expression of GRP78,p-PERK and CHOP proteins were deter-mined by immunohistochemistry and Western blot analysis.Results:Compared with sham group,the neurological deficit score and the brain water content were significantly increased(P<0.05),cerebral infarct size was enlarged(P<0.05),and the expression of GRP78, p-PERK and CHOP were up-regulated in I/R group(P<0.05).At the corresponding time,compared with I/R group,the neurological deficit score and the brain water content were markedly decreased(P<0.05),cerebral infarct size was smaller(P<0.05),the level of GRP78 was notablely increased(P<0.05),while the expression of p-PERK and CHOP were down-regulated in Res group(P<0.05). Conclusion:Resveratrol plays a protection role in ischemia-reperfusion injury, through inhibiting the endoplasmic reticulum stress in rats.
