The inhibitory effect of OSTP-DDP on the growth of ovarian cancer A2780 cells
10.3969/j.issn.1001-1978.2016.02.012
- VernacularTitle:OSTP-DDP靶向抑制卵巢癌A2780细胞生长作用的研究
- Author:
Qiaoran LI
;
Zheng TANG
;
Xuelin LU
;
Xiaomin LIU
;
Xiaoqiu LIANG
- Publication Type:Journal Article
- Keywords:
ovarian cancer cell A2780;
ovarian cancer specific targeting peptide (OSTP) and cisplatin(DDP);
DDP;
growth inhibition;
apoptosis;
targeting therapy
- From:
Chinese Pharmacological Bulletin
2016;(2):204-210
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the growth inhibitory effect of the conjugate ( ovarian cancer specific targeting peptide and cisplatin, OSTP-DDP ) that targeting ovarian cancer cells A2780. Methods Using chemical method to syn-thesize OSTP-DDP, ovarian cancer cells A2780 were cul-tured in vitro, using CCK-8 method ( Cell Counting Kit-8) to detect the growth inhibitory effect of ovarian cancer A2780 cells, which were disposed by OSTP-DDP and DDP. Annexin V-FITC was used to detect the cycle and apoptosis effect of ovarian cancer A2780 cells which were disposed by OSTP-DDP and DDP. Results According to the mass spectrometry and the high performance liquid chromatography ( HPLC ) analysis, OSTP-DDP was proved to synthesize successfully. CCK-8 assay showed that both OSTP-DDP and DDP could play the growth in-hibitory effect and showed a concentration-dependent manner when cells were treated in different concentrations (10,20,40,80,160,320μmol·L-1 ) respectively after 24 h, 48 h, 72 h. And the effect of OSTP-DDP was stronger than DDP (P<0. 05), indicated OSTP-DDP had targeted cytostatic effect. The result of the flow cytometry showed that cell cycle was mostly arrested in G1 phase after 72h treated by OSTP-DDP and DDP, the inhibitory effect of OSTP-DDP was stronger than DDP (P<0. 05). The apop-tosis effect of OSTP-DDP was stronger than DDP ( P <0. 01),suggested that OSTP-DDP had a stronger targeting apoptosis-inducing effect. Conclusion OSTP-DDP has the targeting growth inhibitory effect on the ovarian cancer cell A2780, OSTP as a chemotherapeutic drug targeting vector has a great prospect to treat ovarian cancer.
