Comparison of marker gene expression changes in different mouse models of cardiac hypertrophy
10.3969/j.issn.1001-1978.2016.02.024
- VernacularTitle:小鼠不同心肌肥厚模型标志性基因表达变化的比较
- Author:
Hongwei KAN
;
Wenwen SI
;
Yanyan YIN
;
Can HE
;
Jie CHENG
;
Chunyan WANG
;
Qiongguang ZHANG
;
Yan YANG
- Publication Type:Journal Article
- Keywords:
mice;
cardiac hypertrophy;
ANP;
BNP;
β-MHC;
model
- From:
Chinese Pharmacological Bulletin
2016;(2):274-278
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the differences in hyper-trophic marker genes such as atrial natriuretic peptide ( ANP) , brain natriuretic peptide ( BNP) and β-myo-sin heavy chain (β-MHC) genes in different models of cardiac hypertrophy. Methods Respectively using re-nal abdominal aortic coarctation ( AAC) , arteriovenous fistula ( AVF) and isoproterenol ( ISO) methods to es-tablish C57BL/6 mouse model of cardiac hypertrophy. After modeling, each mouse ’ s body weight ( BW ) , heart weight ( HW) and left ventricular weight ( LVW) were weighed, and the heart weight ( HW/BW) and left ventricular index ( LVW/BW ) were calculated;myocardium by HE staining, pathological morphologi-cal changes were observed; myocardium by immuno-histochemistry, ANP, BNP and β-MHC protein ex-pression was observed;myocardium by Real-time PCR detection, ANP, BNP and β-MHC mRNA expression was observed. Results Compared with control group, HW/BW and LVW/BW were increased in three mod-els. Through the light microscope, each mouse model showed varying degrees of cardiac hypertrophy. ANP, BNP and β-MHC were increased in the protein and mRNA expression. Compared with AAC group, AVF and ISO groups’ myocardial tissue ANP, BNP and β-MHC expression were decreased in the protein and mRNA expression. Conclusions Three cardiac hy-pertrophy models are successful. Cardiac tissue ANP, BNP and β-MHC expression in AAC model exceeds AVF and ISO model.
