Combination of scFv-AFP with doxorubicin inhibits ceII proIiferation in hepato-ceIIuIar carcinoma ceII Huh7
10.11665/j.issn.1000-5048.20160115
- VernacularTitle:抗甲胎蛋白单链抗体与阿霉素联合对人肝癌细胞 Huh7增殖的抑制作用
- Author:
Yanli SHEN
;
Xiaonan JI
;
Xiangdong GAO
- Publication Type:Journal Article
- Keywords:
alpha fetoprotein;
single-chain variable fragment;
doxorubicin;
combination;
hepatocellular carcinoma;
cell proliferation
- From:
Journal of China Pharmaceutical University
2016;(1):101-105
- CountryChina
- Language:Chinese
-
Abstract:
This study was to investigate the inhibitory effects of single-chain variable fragment of alpha fetopro-tein (scFv-AFP)in combination with doxorubicin on the proliferation of human hepatocellular carcinoma cell lines Huh7.Huh7 cells were treated with different concentration of scFv-AFP or doxorubcin alone or their combi-nation.The inhibitory effects were detected by MTT assay,and cycle arrest and apoptosis of Huh7 cells were ana-lyzed by flow cytometry in different groups using PI and Annexin V /PI-staining respectively.Results showed that scFv-AFP,doxorubicin alone or in combinations dose-dependently inhibited the proliferation of Huh7,and a syn-ergistic effect was observed in their combined action.The combination treatment resulted in significantly higher apoptosis than those in other groups (P <0.05).scFv-AFP (40 μg/mL)markedly blocked the Huh7 cell pro-gression by arresting the cells in the G0 /G1 phase,and the percentage of cells in S phase decreased dramatically (P <0.05);and scFv-AFP combined with doxorubicin blocked the Huh7 cell progression by arresting the cells in G2 /Mphase (P <0.01).
