Selection,soluble expression and identification of full human scFvs against IL-13
10.3969/j.issn.1000-484X.2016.01.014
- VernacularTitle:抗IL－13全人源单链抗体的筛选、可溶性表达及鉴定
- Author:
Dengmei ZHANG
;
Siji NIAN
;
Yingchun YE
;
Yan YANG
;
Hong YU
;
Qing YUAN
- Publication Type:Journal Article
- Keywords:
IL-13;
Human scFv;
Phage display
- From:
Chinese Journal of Immunology
2016;(1):65-68
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To select specific and neutralizing scFv against IL-13 and soluble expression and identification them.Methods:In our previous study the scFv library were constructed,and here the scFv library was enriched and the positive scFv were screened from the enriched scFv library for three round.The specific positive scFvs with better affinity were ligated with expression vector for expression and identification.Results:After three rounds of enrichment,30%of clones were positive.The two specific scFvs with better affinity and neutralization were selected from almost 500 clones and then ligated with expression vector LZ16 for soluble ex-pression.The expressed scFvs were identified by western blot and biomolecular interaction analysis.Conclusion: The specific scFvs against IL-13 with better affinity and neutralization had been selected successfully.
