Effect of P53 inhibitors and microtubule inhibitors on nuclear translocation of glucocorticoid receptor in psoriatic epidermal keratinocytes
10.3969/j.issn.1000-484X.2016.01.022
- VernacularTitle:P53抑制剂和微管抑制剂对银屑病表皮角质形成细胞糖皮质激素受体核转运的影响
- Author:
Yuhua ZHANG
;
Wenhui HUANG
;
Jucai MO
- Publication Type:Journal Article
- Keywords:
Psoriasis;
Glucocorticoid receptor;
P53 inhibitor;
Microtubule inhibitors
- From:
Chinese Journal of Immunology
2016;(1):101-103
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of P53 inhibitors and microtubule inhibitors on nuclear translocation of glucocorticoid receptor in psoriatic epidermal keratinocytes.Methods: Isolate and culture psoriatic and normal epidermal keratinocytes.The keratinocytes were incubated with P53 inhibitors and microtubule inhibitors with or without vascular endothelial growth factor( VEGF) ,and then detected the distribution of GR by indirect immunofluorescence.Results:VEGF induced nuclear trans-location of GR in normal keratinocytes, and the P53 inhibitor restrained VEGF induced nuclear export of GR in normal keratinocytes.The nuclear translocation score of the keratinocytes cultured with VEGF was significantly lower than that of keratinocytes cultured without VEGF(P<0.05).The microtubule inhibitors could completely detained GR of normal epidermal keratinocytes in the cytoplasm,and there′s no significantly increased of the level of GR in the cytoplasm after putting VEGF into the normal epidermal kera-tinocytes.While the microtubule inhibitors and P53 inhibitors co-cultured, there will be a small amount of GR into the keratinocyte nuclei.Conclusion:Microtubule mediated uptake of GR,P53 participated nuclear export of GR.
