Let-7a/g/i targeted to Aurora-B in human osteosarcoma cells
10.3969/j.issn.1007-3969.2015.12.008
- VernacularTitle:Let-7a/g/i在骨肉瘤细胞中靶向调控Aurora-B表达
- Author:
Yunfei ZHOU
;
Jiaming LIU
;
Xuanyin CHEN
;
Liangbo ZHU
;
Xinhua LONG
;
Yang ZHOU
;
Zhihong ZHANG
;
Zhili LIU
- Publication Type:Journal Article
- Keywords:
Osteosarcoma;
let-7;
Aurora-B
- From:
China Oncology
2015;(12):966-971
- CountryChina
- Language:Chinese
-
Abstract:
Background and purpose:MicroRNA(miRNA) is a class of small non-coding RNA playing an important regulatory role in many tumors. This study investigated which miRNA might negatively regulate the expression of Aurora-B in osteosarcoma cells, and to lay the foundation for the further investigation of the effort and regulation of Aurora-B in osteosarcoma malignant phenotype.Methods:Bioinformatics prediction software (http://www.targetscan.org) and luciferase assays were used to investigate which miRNA might target to modulate the Aurora-B. Real-time fluorescent quantitative polymerase chain reaction (RTFQ-PCR) and Western blot assay were used to further verify which miRNA could negative regulate the expression ofAurora-B gene.Results:Bioinformatics prediction showed let-7 family have the possibility to modulate the expression of Aurora-B; Luciferase assays showed thatAurora-B might be the target gene of let-7a/b/c/d/e/f/g/i; RTFQ-PCR and Western blot analysis testiifed that both the expression levels of Aurora-B mRNA and Aurora-B protein were signiifcantly decreased in Let-7a/g/i up-regulated U2-OS and HOS cells, compared to the cells in the negative control group; but in Let-7b/c/d/e/f up-regulated U2-OS and HOS cells, the expression levels of Aurora-B mRNA and Aurora-B protein have no signiifcant difference, compared to the cells in the negative control group.Conclusion:Let-7a/g/i may downregulate the expression of Aurora-B in human osteosarcoma cells.
