Preventive Effects of Aspidopterys obcordata on Renal Tubular Epithelial Cells Injury
10.3870/j.issn.1004-0781.2016.03.008
- VernacularTitle:倒心盾翅藤对肾小管上皮细胞损伤的保护作用
- Author:
Meifang SONG
;
Yihang LI
;
Xuelan LI
;
Zhonglian ZHANG
;
Yana LUY
;
Guang LI
- Publication Type:Journal Article
- Keywords:
Aspidopterys obcordata Hemsl.var.obcordata;
Sodium oxalate;
HK-2 cells;
Injury,epithelial cells,renal tubu-lar;
Extract,different solvent
- From:
Herald of Medicine
2016;(3):249-252
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the preventive effects of different extracts from Aspidopterys obcordata on renal tubu-lar epithelial cells injury induced by sodium oxalate in vitro, and initially identify the effective part for treating urolithiasis. Methods The injury model of HK-2 cells induced by sodium oxalate was established to screen the active parts of Aspidopterys obcordata by testing the protective effects of different polarity extracts on HK-2 injury cells through MTT method. Results Dif-ferent extracts from Aspidopterys obcordata improved the activity of HK-2 injury cells, which were elevated to 86.17% and 95.42%by 0. 5 mg?mL-1 and 1 mg?mL-1 aqueous extract, respectively. And the activity reached to 93. 59% and 84. 77% by 0.5 mg?mL-1 and 1 mg?mL-1 50% alcohol extracts, reached above 81.56% by 95% alcohol extracts,all of which showed sig-nificant difference compared with the model group. The HK-2 cells viability were elevated to 82. 53% and 91. 58% by 0.5 mg?mL-1 and 1 mg?mL-1 95% alcohol extracted ethyl acetate parts,and increased to 77.24% and 87.22% by 0.5 mg?mL-1and 1 mg?mL-1 of 95% alcohol isolated n-butanol extracts, approached to 95.46% and 81.36% by 0.5 mg?mL-1 and 1 mg?mL-1 water extracts, all of which showed significant difference compared with the model control group. Conclusion The aqueous extracts and alcohol extracts from Aspidopterys obcordata have obvious preventive effects on HK-2 cells injury, among which the ethyl acetate extracts, n-butanol extracts and water extracts present the remarkable effects, which are supposed to be the active parts for inhibiting calcium oxalate stone formation in vitro.
