Experimental study on inhibitory effect ofβ-elemene on ECV-304 cells'proliferation
- VernacularTitle:β-榄香烯抑制 ECV-304细胞增殖的实验研究
- Author:
Jiuhong MA
;
Hongming JI
;
Rao WEI
;
Jianzhong GUO
- Publication Type:Journal Article
- Keywords:
β-elemene;
Vascular endothelial cells;
Apoptosis;
Angiogenesis
- From:
Journal of Chinese Physician
2015;(z2):5-8
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the growth inhibition of β-elemene in ECV-304 cells,and study it's meaning to anti-angiogenesis in tumors.Methods The IC50 value of β-elemene in ECV-304 cells were got through MTT assay.Under treatment of β-elemene in ECV-304 cell,flow cytometry(FCM)was used to investigated the changing of cell cycle,electron microscope was used to show the morphological al-terations,agarose gel electrophoresis was used to detect effection on cells'DNA.Results The proliferation of ECV-304 cells was significantly inhibited by β-elemene,the IC50 value was (58.3 ±8.1)μg/ml.By FCM,we observed Cell cycle was significantly arrested in G0 /G1 phase under 60μg/ml treatment(P <0.05),apoptotic cells increased with the increase of drug concentrations.Typical apoptotic morphological alterations were found under electron microscope.Characteristic DNA-ladder standing for apoptosis was showed in agarose gel electrophoresis.Conclusions β-elemene can significantly inhibit ECV-304 cells'pro-liferation By inducing apoptosis.
