Inhibition Effects of Rupixiao Granules on Contractility of Uterus of Mice in Vitro
10.3870/j.issn.1004-0781.2016.02.005
- VernacularTitle:乳癖消颗粒对小鼠体外子宫收缩的抑制作用?
- Author:
Lu ZHONG
;
Hongyu JI
;
Yuting ZHUANG
;
Shuyuan WANG
;
Bo CHEN
;
Yuyang SUN
;
Linhua WU
- Publication Type:Journal Article
- Keywords:
Rupixiao granules;
Oxytocin;
Uterine contraction;
Inhibition effects;
Dysmenorrheal,primary
- From:
Herald of Medicine
2016;(2):129-131
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study effects of Rupixiao granules on contraction of uterus of mice in vitro. Methods Mouse model with isolated uterine contraction was established. The mice pretreated with estradiol benzoate were sacrificed and then the uteri were taken out. The normal contractions were recorded by the biological function system. Effects of 10, 20, 30, 40, 50, 60 mg.mL-1 Rupixiao granules on spontaneous and oxytocin-induced (5 U.L-1) uterine contractions were recorded,inhibition of Rupixiao granules on mean constriction amplitude,constriction frequency and contraction activity of pitocin-pretreated uterus was observed and the inhibition rates were calculated. Results Compared with the baselines, the amplitude, frequency, activity of spontaneous uterine contraction were significantly down-regulated by Rupixiao solution at a range of 10-60 mg.mL-1 ,as well as it could inhibit oxytocin-induced spasmodic contraction of isolated uterus at 10-50 mg . mL-1 ( P<0. 01 ) . Conclusion The Rupixiao granules inhibits spontaneous contraction of isolated mouse uteri and oxytocin-induced spasm of uterine smooth muscle in vitro.
