Determination of Gallic Acid and Oleandic Acid in Compound Huanggen Granules by UPLC with Double-wavelength
10.3870/j.issn.1004-0781.2016.02.017
- VernacularTitle:双波长超高效液相色谱法测定复方黄根颗粒中没食子酸和齐墩果酸含量?
- Author:
Hui YANG
- Publication Type:Journal Article
- Keywords:
Compound Huanggen granules;
Gallic acid;
Oleandic acid;
Ultra performance liquid chromatography
- From:
Herald of Medicine
2016;(2):172-174
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an UPLC method for content determination of gallic acid and oleandic acid in compound Huanggen granules. Methods Agilent Eclipse Plus C18 column was used with the mobile phase of methanol-0.1%phosphoric acid by gradient elution. Flow rate was 0.2 mL.min-1 . Detection wavelength was set at 270 nm in the first 7 min for gallic acid and then changed to 215 nm for oleandic acid between 7 and 14 min. Results The linear concentration ranges of gallic acid and oleandic acid were 0.044 1-0.397 1 μg ( r=0.999 6,n=5) and 0.062 1-0.558 7μg ( r=0.999 8,n=5) . Their average recovery was 100. 20% ( RSD=0. 45%) and 99. 96%( RSD=0. 64%) , respectively. Conclusion The extablished method is simple,reproducible and specific, and can be use to the quality control of compound Huanggen granules.
