Expression of microRNA-Let-7a in patients of Non-small cell lung cancer and anti-tumor mechanism
10.3969/j.issn.1000-484X.2016.02.020
- VernacularTitle:microRNA-Let-7 a在肺癌患者中的表达及其抑癌机制的探讨
- Author:
Wenjing XU
;
Dongyun HUANG
;
Ping CHEN
;
Xingxiang XU
- Publication Type:Journal Article
- Keywords:
microRNA-Let-7a;
NSCLC;
Migration;
Proliferation;
k-Ras
- From:
Chinese Journal of Immunology
2016;(2):234-238
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of microRNA-Let-7a in the serum and tumor tissue of non-small cell lung cancer( NSCLC) patients and the effects of cancer cell migration and proliferation.Methods: 50 cases of NSCLC patients in our hospital as the study group,50 healthy volunteers were used as control group,we used Real-time RCR to detect the expression of microRNA-Let-7a in the serum and tumor tissue of NSCLC patients.Using microRNA let-7a mimics transfected into A549,the level of cancer cell migration was observed by transwell,the level of cell proliferation was observed by CCK-8,the level of k-Ras was observed by Real-time RCR and Western blot.Results: The expression of microRNA-Let-7a in the serum and tumor tissue of NSCLC patients was significantly higher than the control group,the difference was statistically significant (P<0.05).After microRNA let-7a transfected into A549,the levels of cancer cell migration and proliferation were significantly decreased,the difference was statistically significant (P<0.05),the mRNA and protein levels of k-Ras were reduced inA549 cells,the difference was statistically significant (P<0.05). Conclusion:The expression of microRNA let-7a is low in the serum and tumor tissue of NSCLC patients,and may weaken the levels of cancer cell migration and proliferation through the Ras signaling pathway.
