Analysis of quantity and function of dendritic cells in peripheral blood of patients with repeated implantation failure
10.3969/j.issn.1000-484X.2016.02.021
- VernacularTitle:反复种植失败患者周围血树突状细胞数量和功能分析
- Author:
Yong ZENG
;
Chunyu HUANG
;
Xian CHEN
;
Peiyan LIANG
;
Lianghui DIAO
;
Cong CHEN
;
Xu ZHANG
;
Biao YIN
- Publication Type:Journal Article
- Keywords:
Maternal-fetal immune tolerance;
Repeated implantation failure;
Peripheral blood;
Dendritic cells
- From:
Chinese Journal of Immunology
2016;(2):239-243
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the quantity and function changes of dendritic cells ( DC) in peripheral blood of patients with repeated implantation failure ( RIF).Methods:30 patients with RIF and 15 normal controls were enrolled in this study,and the peripheral blood was collected during the mid-luteal phase.The percentage of DC subsets and the expression levels of functional molecules were assessed by flow cytometric analysis.Results:Compared with normal controls,the percentage of lin-HLA-DR+DC cells accounting for leukocytes in patients with RIF was not significantly different ( P>0.05).There were also no significant differences in the expression levels of co-stimulatory molecules ( CD80 and CD86) and immune tolerant molecules CD200 on DC cells surfaces between patients with RIF and normal controls ( all P>0.05).In addition,the percentage of CD11c+CD123-mDC accounting for DC cells was significantly increased in patients with RIF (P<0.05),however,the percentage of CD11c-CD123+pDC was similar (P>0.05). Conclusion:The percentage of mDC accounting for DC cells was significantly increased in patients with RIF, which may be one of factors affecting pregnancy outcomes.
