Effect of aspirin on somatostatin receptors level in SD rat′s intestinal mucosa
10.3969/j.issn.1000-484X.2016.02.007
- VernacularTitle:阿司匹林对SD大鼠小肠黏膜生长抑素受体水平的影响
- Author:
Tianfei YANG
;
Tong ZHEN
;
Qinghua TAN
;
Zhuping CHE
- Publication Type:Journal Article
- Keywords:
Intestinal Mucosa;
Aspirin;
SSTR1-5;
Approach
- From:
Chinese Journal of Immunology
2016;(2):178-183
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the optimal administration approach of using aspirin by observing the effects of the rats in-testinal mucosal barrier and somatostatin receptors level with different administration approaches of using non-steroid anti-inflammatory drugs-aspirin.Methods:32 SD rats were randomly divided into 4 groups:oral intake group,enema group,intraperitoneal injection group and control group.Every group had 8 rats.The ileum mucosal injury was scored.The level and distribution of SSTRs in every group was detected by ELISA and immunohistochemistry, respectively, The IOD score in each group was measured by image analysis.Results:(1) The scores of ileum mucosal injury in aspirin experimental groups were significantly higher than that in control group (P<0.05), but there were no difference among aspirin experimental groups.( 2 ) All subtypes of SSTR were expressed in rat intestinal mucosa.However,SSRT1 and SSTR2 were expressed mainly ( P<0.05 ).( 3 ) The expressions of SSTR1-5 on intestinal mucosa in aspirin experimental groups were significantly less than that in control group (P<0.05).(4) There was incompletely no same effects of SSTR1-5 in intestinal mucosa by different approaches ( P<0.05 ).( 5 ) There were no significant difference of SST concentration and IOD score among all groups ( P>0.05).Conclusion:Aspirin can damage the rat intestinal mucosa.Aspirin could lead to the decrease of SSTR1-5 expression in the intestinal mucosa.All subtypes of SSTR in rat small intestine mucosa were expressed, suggesting that aspirin could affect the barrier function of the intestine.There were different influences on SSTR1-5 expression by different administration approaches,suggesting aspirin by parenteral approach may reduce the intestinal damage.
