Research of astragalus polysaccharides increasing immune effect of influenza A virus HA2 eukaryotic expression vector

VernacularTitle:黄芪多糖对甲流HA2蛋白真核表达载体免疫效果影响的研究
Author: Zhaoping CHU ; Shuhui WU ; Wentai LIU ; Zhihong MA ; Bingyuan XU ; Jun LUO ; Gang CAO ; Huazhou XU ; Yue SHI ; Jun DAI
Publication Type:Journal Article
Keywords: Influenza A virus; Hemagglutinin; IFN-γ; IL-4
From: Chinese Journal of Immunology 2016;(2):189-192
CountryChina
Language:Chinese
Abstract: Objective:To study the astragalus polysaccharides ( APS) effect on immune induced by influenza A virus HA2 eu-karyotic expression vector.Methods: The HA2 encoded by the DNA vaccine vector was efficiently expressed in CHO cells, as determined by reverse transcription polymerase chain reaction ( RT-PCR) and fluorescence analysis.60 rats were divided into six groups randomly,which were immunized with normal saline,pEGFP-N1,pHA2/EGFP+different dose of APS by intramuscular injection.The control sera were collected before injection.After injected the 36th day, sera were collected to analyzing IFN-γ, IL-4 and IgG level.Results:IFN-γ,IL-4 and IgG level of pHA2/EGFP+mAPS group was different from that of pEGFP-N1 group or pHA2/EGFP+lAPS group( P<0.05 ).Conclusion: Middle dose of APS could increase immune induced by influenza A virus HA2 eukaryotic expression vector.