Effcets on immunoregulation of iNKT cells in RA by novel synthetic immunos-timulator CH1 b
10.3969/j.issn.1000-484X.2016.02.016
- VernacularTitle:含噻唑烷-4-酮的免疫调节剂对RA患者iNKT细胞免疫调节功能的影响
- Author:
Ming MENG
;
Xuejiao ZHANG
;
Peishan WENG
;
Minghua XU
;
Dan CHEN
;
Minghui HOU
;
Dongzhi CHEN
- Publication Type:Journal Article
- Keywords:
Rheumatoid arthritis( RA);
Invariant nature killer T( iNKT);
Immunostimulants;
IFN-γ/IL-4
- From:
Chinese Journal of Immunology
2016;(2):218-222
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate effects of a novel synthetic immunostimulator CH1b containing thiazolidin-4-one on the immunoregulation funotion of iNKT ( invariant nature killer T ) cells in active RA patients in vitro.Methods: Peripheral blood mononuclear cells( PBMCs) isolated from active RA patients were cultured with stimulation of α-Galcer and IL-2 in vitro and iNKT cells were then separated by using magnetic activated cell sorting( MACS) method with iNKT isolation kit.The cells were divided into three groups:control group (IL-2),α-Galcer group (IL-2+α-Galcer),CH1b group(IL-2 +CH1b).The effects of CH1b on the proliferation of iNKT cells in active RA patients were analyzed by using MTT assay.MILLIPLEX MAP Human Cytokine/Chemokine kit was used to evaluate the secretion of IFN-γand IL-4 in iNKT cells culture media.The expressions of IFN-γmRNA and IL-4 mRNA in iNKT cells were analyzed by RT-PCR.Results: Compared with control and α-Galcer group,the proliferation of iNKT cells of CH1b group were significantly higher( P<0.05).Compared with control,the ratio of IFN-γ/IL-4 in iNKT cells culture media in active RA patients of CH1b group were significantly lower (P<0.05).Compared with control,expressions of IFN-γmRNA and IL-4 mRNA were higher inα-Galcer group;compared with control,expressions of IL-4 mRNA were higher in CH1b group,while there were no obvious difference on expressions of IFN-γmRNA.Conclusion:CH1b was found to significantly stimulate the actived iNKT cells in active RA patients proliferation,promote the secretion of IL-4,and increase the ratio of IFN-γ/IL-4,promote the expression of IL-4 mRNA in iNKT cells in active patients.
