Studies on immunogenicity and immunoprotection induced by heat shock protein 60 kDa of Schistosoma japonicum in mice
10.16250/j.32.1374.2015249
- VernacularTitle:日本血吸虫热休克蛋白60 kDa的免疫原性及保护性研究
- Author:
Xin JIN
;
Xiaojun CHEN
;
Jifeng ZHU
;
Zhipeng XU
;
Feng LIU
;
Sha ZHOU
;
Chuan SU
- Publication Type:Journal Article
- Keywords:
Schistosoma japonicum;
Heat shock protein 60 kDa;
Immunoprotection;
CD4+CD25+ regulatory T cells
- From:
Chinese Journal of Schistosomiasis Control
2016;(1):45-50
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the immunogenicity and immunoprotective effect of heat shock protein 60 kDa (SjHSP60) of Schistosoma japonicum in mice after immunization and challenge infection, and explore the mechanism. Methods B cell/an?tibody?related databases and analysis tools were used to predict B?cell epitopes of SjHSP60. The mice were immunized with the recombinant SjHSP60 and challenged with S. japonicum cercariae. SjHSP60?specific antibodies in serum were detected by ELI?SA. The level of splenocyte proliferation was determined by 3H?TdR incorporation. Ex vivo suppression assay was performed to in?vestigate the effects of CD4 +CD25 + regulatory T cells (Tregs) induced by SjHSP60. Results SjHSP60 possessed multiple pre?dominant regions of B?cell epitopes. SjHSP60 induced a significant increase in both SjHSP60?specific IgG levels (P < 0.01) and splenocyte proliferation (P < 0.01) with a higher IFN?γ production (P < 0.01). However, the immunization with SjHSP60 resulted no significant reduction in adult worms (P > 0.05) and liver?accumulated eggs (P > 0.05) in S. japonicum?infected mice. Ex vivo assay showed that CD4+CD25+ Tregs from SjHSP60?immunized mice enhanced immunosuppressive activity. Conclusion SjH?SP60 has a dual role in host immune system, being involved in the induction of dominant humoral and cellular immune responses as well as in the enhancement of immunosuppression.
