Activity identification of Streptococcus suis suilysin inducing platelets aggregation
10.7644/j.issn.1674-9960.2016.01.010
- VernacularTitle:猪链球菌溶血素（SLY）引起血小板聚集活性分析
- Author:
Shengwei ZHANG
;
Peng LIU
;
Maokai XU
;
Xueyi SHANG
;
Yuling ZHENG
;
Yuan YUAN
;
Yongqiang JIANG
- Publication Type:Journal Article
- Keywords:
Streptococcus suis serotype 2;
suilysin;
platelets aggregation;
aspirin;
thrombocytopania
- From:
Military Medical Sciences
2016;(1):40-44
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the interaction of streptococcus suis serotype 2 recombinant suilysin ( SLY ) with platelets, and provide the theoretical basis for clinic treatment of patients infected with S.suis.Methods The nickel column affinity chromatography was used to purify the recombinant SLY.The hemolytic acivity was identified by optical density before the platelets aggregation induced by a SLY was detected by a platelet aggregometer or electron microscope and the effect of aspirin on platelets aggregation was analyzed.The impact of wild type 05ZYH33 and sly-deficient mutant strainΔSLY on platelets of mice was compared to predict the interaction of the SLY with platelets in vivo.Results and Conclusion Hemolytic activity of recombinant SLY was 2000 hemolytic units( HU) and platelets aggregation was induced at 1 μg/ml.The aggregation can be inhibited by aspirin in 5 mmol/L.SLY can also increase the volume and reduce the amount of platelets in mice.
