Roles of Tim-3 in paraquat poisoning induced acute inflammatory response
10.7644/j.issn.1674-9960.2016.01.011
- VernacularTitle:百草枯中毒急性炎症反应中Tim-3的调控作用研究
- Author:
Jiahui YU
;
Chunmei HOU
;
Xingwei JIANG
;
Wei WANG
;
Qingzhu SHI
;
Guojiang CHEN
;
Renxi WANG
;
He XIAO
;
Jiannan FENG
;
Beifen SHEN
;
Yan LI
;
Yuanfang MA
;
Gencheng HAN
- Publication Type:Journal Article
- Keywords:
Tim-3;
paraquat;
poisoning;
inflammatory mediators;
tumor necrosis factorα;
interleukin
- From:
Military Medical Sciences
2016;(1):45-50
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine whether Tim-3 plays a protective role in paraquat poisoning induced excessive immune response and tissue damage based on the critical roles of Tim-3 controlling inflammatory response.Methods A paraquat poisoning model was established in wild type and in Tim-3 transgenic C57BL/6 mice by intraperitoneal injection of paraquat (40 mg/kg) .In addition, C57BL/6 mice with paraquat poisoning were injected with Tim-3 soluble protein( sTim-3) or control protein to see the effect of Tim-3 blocking on the progression of paraquat poisoning.Samples were collected at 6 and 24 h after paraquat injection respectively and were examined for tissue damage, cytokine expression and paraquat metabolism.Results After paraquat poisoning, there was significantly attenuated tissue damage in the lungs and kidneys and decreased TNF-α,IL-6 and IL-1 beta expression in the PBMCs or in the serum from Tim-3 transgenic mice compared to wild type mice.The serum concentration of paraquat in Tim-3 transgenic mice was also significantly decreased.However, in sTim-3 treated paraquat poisoning mice, there was significantly increased cytokine expression and tissue damage compared to control protein treated mice.The in vitro data showed that Tim-3 signaling negatively regulated macrophages mediated inflammatory response.Conclusion Tim-3 plays a critical role in maintaining the homeostasis after paraquat poisoning. Further investigation on the regulatory roles of Tim-3 in inflammation will shed new light on the pathogenesis of paraquat poisoning and provide new therapeutic strategies.
