Lentivirus-mediated knockdown of human E6 AP inhibits gastric cancer cell prolifera-tion and migration
10.7644/j.issn.1674-9960.2016.01.012
- VernacularTitle:慢病毒介导的敲低E6 AP表达抑制胃癌细胞增殖和迁移
- Author:
Jiaxiu XIONG
;
Zhida CHEN
;
Yanan ZHANG
;
Xiaomeng JIA
;
Yingjie CHEN
;
Youhua XIONG
;
Lihua DING
;
Qinong YE
;
Wenzhong LIU
;
Bo WEI
- Publication Type:Journal Article
- Keywords:
E6AP;
lentivirus;
RNA interference;
gastric neoplasms;
cell proliferation
- From:
Military Medical Sciences
2016;(1):51-54
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the effect of E6AP on gastric cancer cell proliferation and migration.Methods The expression of E6AP in different gastric cancer cell lines and normal gastric mucosa epithelial cell lines was detected by Western blotting.Gastric cancer cells BGC-823 stably expressing E6AP short hairpan RNA(shRNA) were obtained by lentiviral vector of E6AP.The effect of E6AP on BGC-823 cell growth and migration was determined by CCK-8 kit, Tran-swell and wound healing assay.Results Gastric cancer cell line BGC-823 in which E6AP was stably knocked down was established.Knockdown of E6AP inhibited the proliferation and migration of BGC-823 cells.Conclusion E6AP plays a key role in gastric cancer proliferation and migration.
