Application of ATP bioluminescence assay in survey of cleanliness of hos-pital environmental object surfaces and health care workers’hands
10.3969/j.issn.1671-9638.2016.01.013
- VernacularTitle:应用ATP生物荧光法调查医院环境物体表面及医务人员手清洁度
- Author:
Fangfei LIU
;
Jinna LI
;
Xiaojing HE
;
Yao SUO
;
Hongmei WANG
;
Ningning WANG
;
Xiaoling FANG
- Publication Type:Journal Article
- Keywords:
ATP bioluminescence assay;
object surface;
ventilator;
health care worker;
hand hygiene;
cleaning;
disinfection
- From:
Chinese Journal of Infection Control
2016;(1):52-55
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate cleanliness of hospital environmental object surfaces and hands of health care workers(HCWs).Methods The adenosine triphosphate (ATP)bioluminescence assay was used to detect object surfaces and hands of HCWs in a hospital,on-the-spot intervention was conducted.Results The qualified rates of hospital environmental object surfaces and ventilator-relevant object surfaces were 58.14% (200/344)and 69.88%(116/166)respectively,the qualified rate of ventilator tracheal intubation site was low (29.17%);the qualified rate of telephone surfaces was the lowest (27.27%).The qualified rates of ventilator-relevant object surfaces used con-tinuously for ≥48h and <48 h were 56.70%(55/97)and 88.41 %(61/69)respectively,there was significant differ-ence between the two(χ2 =19.26,P <0.01).The qualified rates of HCWs’hands before and after intervention were 34.18% and 85.58% respectively,relative light unit (RLU)values were (1 033.46±106.20)and (80.46±10.68) respectively,the qualified rates and RLU before and after intervention were both significantly different (both P <0.01).Conclusion Contamination of object surfaces and hands’of HCWs in hospital dynamic environment is seri-ous,ATP bioluminescence detection and on-the-spot intervention is helpful for improving cleanliness of hospital en-vironment object surfaces and HCWs’compliance to hand hygiene.
