Application of mouse anti AEG-1 monoclonal fluorescent antibody in malignant serous cavity effusions
10.3969/j.issn.1673-4130.2016.03.001
- VernacularTitle:鼠源性抗星形胶质细胞上调基因1单克隆荧光抗体在恶性浆膜腔积液检测中的应用
- Author:
Man LU
;
Min LONG
;
Chong LIU
;
Xi CHEN
;
Chen WANG
;
Huizhong ZHANG
- Publication Type:Journal Article
- Keywords:
mouse astrocytes elevated gene-1;
monoclonal antibodies;
fluorescently labeling;
effusion
- From:
International Journal of Laboratory Medicine
2016;(3):289-290
- CountryChina
- Language:Chinese
-
Abstract:
Objective To use mouse astrocytes elevated gene‐1 (AEG‐1) monoclonal fluorescent antibody for detecting tumor cells in malignant serous cavity effusions .Methods The expression of AEG‐1 in serous cavity effusion exfoliated cells by PCR and Western‐blot ;the mouse monoclonal anti‐AEG‐1 fluorescent antibody and tumor cells in malignant serous cavity effusions were co ‐incubated ,meanwhile ,the serous cavity effusions in benign lesions were taken as the negative control .Results The AEG‐1 expres‐sion was positive in malignant serous cavity effusions exfoliated cells ,while which in benign lesion serous cavity effusion was nega‐tive or weakly positive ;meanwhile the results of direct labelling in mouse anti AEG‐1 monoclonal antibodies were consistent with the results by PCR and Western‐blot .Conclusion Mouse anti AEG‐1 monoclonal fluorescence antibody can provide certain theoret‐ical basis for the detection of tumor cells in serous cavity effusion .
