Construction and characterization of invasion protein B gene deleted mutant asd host-vector balanced lethal system
10.3969/j.issn.1000-484X.2016.02.014
- VernacularTitle:鼠伤寒沙门菌SL1344株侵袭性蛋白B缺失株asd平衡致死系统的构建及特性的研究
- Author:
Chunjie ZHANG
;
Songbiao CHEN
;
Xiangchao CHENG
;
Chengshui LIAO
;
Jing LI
;
Lei HE
;
Mingliang ZHANG
;
Chuan YU
;
Zuhua YU
;
Yanyan JIA
;
Zhanqin ZHAO
- Publication Type:Journal Article
- Keywords:
Suicide plasmid;
Host-vector balanced lethal system;
SL1344ΔsipBΔasd( pYA3493);
Oral live vaccine vector
- From:
Chinese Journal of Immunology
2016;(2):210-213
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To develop an oral live vaccine vector which stably carries exogenous genes.Methods:SL1344ΔsipBΔasd host-vector balanced lethal system was constructed by the method of recombinant suicide plasmid-mediated allelic exchange on the basis of attenuated Salmonella typhinurium SL1344ΔsipB.Then,the biological characteristics of SL1344ΔsipBΔasd was analyzed.Results:The results showed that the mutant was stabile with the Δasd gene in vitro;the serotype and growth rate of SL1344ΔsipBΔasd strain was almost same as the parent SL1344ΔsipB and SL1344 strain.And the mutant strains remain swim ming zones.Virulence test in mice showed that the virulence of SL1344ΔsipBΔasd which carried complementary plasmid pYA3493 by electro-transformation decreased by 1.4%compared with SL1344.Conclusion: These results showed that the SL1344ΔsipBΔasd mutant was successfully constructed.It is likely that this mutant should be used as a live vector to express foreign genes.
