Docosahexaenoic acid protects human retinal pigment epithelial cells against oxidative stress-induced apoptosis
10.3969/j.issn.1000-4718.2016.03.019
- VernacularTitle:二十二碳六烯酸抑制氧化应激状态下人视网膜色素上皮细胞凋亡
- Author:
Yuefeng LIU
;
Weimin LUO
;
Yong ZHANG
;
Xiaodong ZHONG
- Publication Type:Journal Article
- Keywords:
Docosahexaenoic acid;
Retinal pigment epithelial cells;
Heme oxygenase-1
- From:
Chinese Journal of Pathophysiology
2016;32(3):504-509
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe the effect of docosahexaenoic acid ( DHA) on H2 O2-induced apoptosis in human retinal pigment epithelium cells and its molecular mechanism .METHODS: Human retinal pigment epithelium cell line ARPE-19 was cultured in vitro, and 12.5 mmol/L H2 O2 was used to mimic the oxidative stress condition .The cells were treated with 30~100μmol/L DHA for 4~24 h.The expression of heme oxygenase-1 (HO-1) at mRNA and protein levels was detected by real-time PCR and Western blot , respectively .The enzymic activity of HO-1 was measured by colorimetry . Production of reactive oxygen species ( ROS) was determined by fluorescent probe .Activation of NF-E2-related factor 2 (Nrf2) was examined by immunofluorescence method .Apoptosis of ARPE-19 cells was analyzed by flow cytometry .RE-SULTS:The mRNA and protein expression and the enzymic activity of HO-1 were significantly increased in the ARPE-19 cells after DHA treatment .Meanwhile , nuclear translocation of Nrf 2 was also observed .Apoptosis appeared and ROS was produced upon H2O2 incubation.In contrast, DHA at 100 μmol/L significantly abrogated H2O2-induced apoptosis and ROS production.Furthermore, silencing of HO-1 by specific siRNA, or treatment with ZnPP, an inhibitor of HO-1, partly counteracted the protective effect against H 2 O2-induced apoptosis and ROS production .CONCLUSION: DHA protects retinal pigment epithelial cells against oxidative stress via induction of heme oxygenase -1 expression after Nrf2 activation .
