Effect of Raloxifene on Cadmium Chloride-Contaminated Renal Tubular Epithelial Cells
10.3870/j.issn.1004-0781.2016.02.003
- VernacularTitle:雷洛昔芬对氯化镉染毒肾小管上皮细胞的影响?
- Author:
Min XIAO
;
Yousheng YIN
;
Bin LENG
;
Qingchu LI
;
Kanghui LI
;
Jiazhi WEI
- Publication Type:Journal Article
- Keywords:
Raloxifene;
Cadmium;
Estrogen receptor;
Metallothionein;
Renal tubular epithelial cell
- From:
Herald of Medicine
2016;(2):120-124
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of raloxifene on ERα, ERβ and MT-1a expression in renal tubular epithelial cells contaminated with cadmium chloride,for exploring the protective effect and action mechanism of raloxifene in renal injury induced by cadmium. Methods Renal tubule cells were isolated from kidneys of new born SD rats and purified by Percoll. The cells of the second generation were selected for experiment. The cells were divided into 3 groups:blank control group,cadmium chloride group and raloxifene group. After 4 h,cell viability was detected by MTT,and after 24 h,mRNA and protein expression levels of ERα, ERβ and MT-1a in renal tissues were determined by RT-PCR and immunohistochemistry technology,respectively. Results Compared with the blank control group,morphology of plenty cells were changed in cadmium chloride group,a number of cells were dead,and the survival rate was only 55.3%;at the same time,mRNA and protein expression levels of ERβ and MT-1a significantly increased (P<0.05). Compared with cadmium chloride group,only a small part of cells underwent morphological changes,and the survival rate was 88.6% in raloxifene group,and the mRNA and protein expression levels of ERβand MT-1a were significantly decreased ( P<0.05) . The expression of ERαshowed no significant difference among the three groups ( P>0.05) . Conclusion Raloxifene can bond with ERβcompetively,down-regulate MT-1a and decrease Cd-MT synthesis,so as to reduce cadmium-induced damage of renal tubular epithelial cells.
