Study on immunological effect of dendritic cell transfected by recombinant plasmid ofΔNp73αon breast cancer
10.3969/j.issn.1000-484X.2016.02.005
- VernacularTitle:Np73α基因转染树突状细胞抗乳腺癌细胞的免疫效应
- Author:
Yang WANG
;
Xiaoqing MIAO
;
Shuhua LI
;
Limei AI
;
Pei ZHANG
- Publication Type:Journal Article
- Keywords:
ΔNp73α;
DC;
CTL;
Vaccine
- From:
Chinese Journal of Immunology
2016;(2):169-173
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To research the imumunological effect of dendritic cell transfected by recombinant plasmid of ΔNp73αon breast cancer.Methods:Cultivate dendritic cells by cytokines( GM-CSF,IL-4,TNF-α) from human umbilical cord blood in vitro, CD1a,CD83 were detected by flow cytometry.Recombinant plasmid pcDNA-HA/ΔNp73αwas used to transfected DCs,the transfection was detected by Western blot.Transfected DCs cocultivate with T cell induce specific cytotoxic lymphocytes( CTL).Proliferation ability of T cell was detected by MTT methods.IFN-γin the culture supernatant of the transfected DCs were detected by ELISA.The killing effect on breast cancer MDA-MB-231 was detected by LDH method.Results:The expression of CD1a(56%),CD83(74%)in mature DC was higher than immature DC(CD1a 19%,CD83 13%)(P<0.01).ΔNp73αtransfected group had a special band.The killing effect of specific CTL induced byΔNp73α-DC and T cells cocultivation on breast cancer cell MDA-MB-231 was statistic stronger than DC group(P<0.05),the proliferation of T cell and secretion level of IFN-γcompared with DC and pcDNA group were statistically significant(P<0.01).Conclusion:The CTL induced byΔNp73αtransfected DC vaccine has significant effect on killing breast cancer.
