Effect of protoscolex on T subsets in mice spleen cells in vitro
10.3969/j.issn.1000-484X.2016.02.006
- VernacularTitle:原头蚴对体外培养小鼠脾细胞中Th细胞亚群的影响
- Author:
Hairui FANG
;
Hongqun JIANG
;
Fangjie XU
;
Jun HOU
;
Dan DONG
;
Congzhe CHEN
;
Xiangwei WU
;
Xueling CHEN
- Publication Type:Journal Article
- Keywords:
Protoscolex;
Spleen cells;
Th cells
- From:
Chinese Journal of Immunology
2016;(2):174-177
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of protoscolex on Th subsets and correlative cytokine in mice spleen cells in vitro.Methods:Co-culture spleen cells from BALB/c mice with protoscolices,then IL-4,IFN-γand TGF-βproduction in cell culture supernatants were analyzed by ELISA.The percentage of Th subsets were detected by Flow Cytometry analysis.Results:Secretion levels of IL-4 and TGF-βwere significantly increased in spleen cells at different time point in co-culture system with protoscolices.Ratios of Th2 and Treg cells were also significantly increased in co-culture system at different time points than the control groups.However,there was no statistical significance for ratio of Th1 cells at different time points.Conclusion:The protoscolex can increase the ratios of Th2 cells and Treg cells from spleen cells.Secretion levels of IL-4 and TGF-βwere also increased in spleen cells co-cultured with protosco-lices.The results suggest that these Th cell subsets play a role in the immune escape of the hydatid disease.
