Effects of minocycline on cerebral blood flow and endothelin-1 protein expression in ischemic penumbra of rats with focal cerebral ischemia reperfusion
10.3969/j.issn.1671-8348.2016.01.010
- VernacularTitle:米诺环素对大鼠脑缺血半暗带脑血流量及内皮素-1蛋白表达的影响
- Author:
Tao TAO
;
Linwang GAN
;
Jie FU
;
Zuoxiao LI
;
Xiaogang LI
- Publication Type:Journal Article
- Keywords:
minocycline;
reperfusion injury;
cerebral blood flow;
endothelin-1
- From:
Chongqing Medicine
2016;(1):27-29
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of minocycline on regional cerebral blood flow and the expression of endothe-lin-1(ET-1) in ischemic penumbra of rats after focal cerebral ischemia reperfusion injury .Methods Middle cerebral artery occlusion (MCAO) with nylon suture was used to be established as focal cerebral ischemia reperfusion mode (I/R) ,a total of 35 male Spra-geue-Dawley rats were randomly divided into 3 groups :sham-operated group(n= 10) ,model group(n= 15) and minocycline group (n= 15) .After 24 hours of I/R ,The neurobehavioral function of rats was evaluated by Longa′s test ,the regional cerebral blood flow in ischemic penumbra was assessed with laser-Doppler flowmetry .After 6 and 24 hours of I/R ,the expression of ET-1 in peri-in-farct region was measured by both immunohistochemistry and radioimmunoassay .Results Compared with sham-group ,the Longa′s test scales ,ET-1 protein expression increased and the rCBF decreased in ischemic penumbra in model group(P< 0 .05) .the Longa′s test scales ,ET-1 protein expression decreased and the rCBF decreased in ischemic penumbra in minocycline group when compared to the model group(P< 0 .05) .Conclusion Minocycline could promote neurological functional recovery of rats after MCAO ,which might be attributed to increase the cerebral blood flow and regulate the endothelin-1 expression in ischemic penumbra .
