Effects of imidapril on blood gas, oxidative stress and inflammatory fac-tors in rats with acute lung injury induced by ammonium chloride
10.3969/j.issn.1000-4718.2016.02.015
- VernacularTitle:咪达普利对氯化铵所致急性肺损伤大鼠血气、MDA及 AngⅡ、CD54蛋白表达的影响
- Author:
Congfu HUANG
;
Xueqin HAO
;
Wen DENG
;
Chuanfa LIU
;
Tingting WANG
;
Xiaoli WEN
;
Zhongrong TAO
- Publication Type:Journal Article
- Keywords:
Imidapril;
Acute lung injury;
Ammonium chloride
- From:
Chinese Journal of Pathophysiology
2016;32(2):278-283
- CountryChina
- Language:Chinese
-
Abstract:
AIM:In this study, the rat lung injury model was induced by ammonium chloride for studying the effect of imidapril on blood gas, serum TNF-α, IL-6 and MDA concentrations, and AngⅡand CD54 protein expression in rat lung tissue.METHODS:Male rats were randomly divided into 3 groups:control group, lung injury model group and drug group.The rats in control group were given saline (2 mL/kg), while the rats in lung injury model group were given 6% ammonium chloride (2 mL/kg).In drug group, imidapril (3 mg· kg-1· d-1) was given to the rats once daily for 1 week by intragastric gavage after given 6%ammonium chloride.On the 7th day, the rats were anesthetized with 2% so-dium pentobarbital.Abdominal aorta blood, venous blood and lung tissue were collected.The blood gas indexes and serum TNF-α, IL-6 and MDA concentrations were determined.The lung tissues were fixed and sliced, and the expression of AngⅡ and CD54 proteins was detected by immunohistochemistry.RESULTS: The PaCO2 increased in lung injury model group compared with control group and drug group (P<0.05).The expression of AngⅡand CD54, and the concentrations of TNF-α, IL-6 and MDA also increased significantly ( P<0.01) in model group.Pulmonary edema, inflammation, alveo-lus congestion, hemorrhage and hyperplasia in model group were obvious compared with control group and drug group. CONCLUSION:Imidapril improves blood gas indexes, and reduces lipid peroxidation and inflammatory responses in the rats with lung injury induced by ammonium chloride.
