Neuroprotective effect of H2 S by inhibiting autophagy after restoration of spontaneous circulation in rats with cardiac arrest
10.3969/j.issn.1000-4718.2016.02.016
- VernacularTitle:硫化氢通过抑制自噬减轻心脏停搏后脑损伤
- Author:
Hongyan WEI
;
Hengjie LI
;
Fang LI
;
Chunlin HU
;
Xin LI
;
Hui LI
;
Ziran ZHAO
;
Jie ZHANG
;
Xiaoxing LIAO
- Publication Type:Journal Article
- Keywords:
Hydrogen sulfide;
Cardiac arrest;
Cardiopulmonary resuscitation;
Autophagy;
Neurons
- From:
Chinese Journal of Pathophysiology
2016;32(2):284-289
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the neuroprotective effect of hydrogen sulfide ( H2 S) after cardiopulmonary re-suscitation in rats with cardiac arrest ( CA) , and to explore the effects of H2 S on neuron autophagy.METHODS:The CA model was established through asphyxia.Male Wistar rats were randomly divided into sham group, model group and NaHS group.The levels of beclin-1 and LC3 II/I were measured by Western blot at 2 h, 4 h, 12 h and 24 h after the restoration of spontaneous circulation (ROSC).At 12 h after ROSC, the formation of autophagic vacuole with LC3 dots was deter-mined by immunohistochemical ( IHC) method.The phenomenon of neuron autophagy was observed under transmission electron microscope.The numbers of apoptotic neurons were counted by TUNEL staining at 72 h after ROSC.The neurolo-gic deficit score ( NDS) was used to evaluate the neurologic function after ROSC.RESULTS: The level of beclin-1 was gradually increased in model group, but it was increased and then gradually recovered in NaHS group ( P<0.05 ) .The conversion of LC3 II in the cerebral cortex was the same as beclin-1.The results of IHC showed that LC3-positive nuclei in model group were more than those in NaHS group ( P<0.05) .The number of autophagic vacuole in model group was more than that in NaHS group (P<0.05).The number of the TUNEL-positive cells in model group was more than that in NaHS group (P<0.05).The NDS of the animals in NaHS group after ROSC was lower than that in model group(P<0.05). CONCLUSION:H2 S inhibits neuronal autophagy, decreases apoptosis and improves neurologic function in CA rats after ROSC.
