Analysis of pulmonary surfactant protein B exons sequencing in 80 very low birth weight prematures
10.3760/cma.j.issn.2095-428X.2016.02.005
- VernacularTitle:极低出生体质量早产儿80例肺泡表面活性物质蛋白B外显子测序分析
- Author:
Feitong ZHANG
;
Qiliang CUI
- Publication Type:Journal Article
- Keywords:
Very low birth weight premature;
Pulmonary surfactant protein B;
Exon;
Sequencing
- From:
Chinese Journal of Applied Clinical Pediatrics
2016;(2):97-100
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship between exon gene polymorphism of pulmonary surfactant pro-tein B(SP -B)and the susceptibility and severity,prognosis of respiratory distress syndrome (RDS).Methods To detect the gene sequence of SP -B exon by adopting the gene sequencing technology,and samples were 80 prematures of very low birth weight in Southern Han Chinese,who were divided into 2 groups,the RDS and the non RDS,and the difference of genotype in SP -B exon in 2 groups was compared.Results There was no significant difference between 2 pretem groups in the aspects of the gestational age,sex,birth weight and delivery mode etc(all P >0.05).Fifty -nine prematures of very low birth weight had exons heterogenesis,and there were 2 types of mutations,V1 :Exon2:c.[5A >C]+[5A >C]or c.[5A >C]+[=];V2:Exon5:c.[428C >T]+[428C >T]or c.[428C >T]+[=].There were 20 cases of type V1 ,1 8 cases of type V2,3 cases of type V1 +V2 in 45 cases of RDS,and there were 1 2 cases of type V1 ,9 cases of type V2,no case of type V1 +V2 in the non RDS group.Comparing the incidence of V1 and V2 in 2 groups,there were all significant differences(χ2 =3.73,5.02;all P <0.05),and the OR values of 2 polymorphisms to RDS were 3.33 and 4.00,but there was no significant difference in mutation cases and non mutation cases in RDS group when comparing severity and prognostic(χ2 =0.07,P >0.05).Conclusion Gene polymorphism of SP -B exon are risk factors for premature of very low birth weight in southern Han Chinese in RDS.
