Preparation of reference materials of total thyroxine

Weiyan Zhou; Songlin YU; Donghuan Wang; Jing Wang; Jie Zeng; Tianjiao Zhang; Jiangtao Zhang; Feng Zhao; Nan Qin; Qinghui Meng; Mo Wang; Wenxiang Chen; Chuanbao Zhang

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Preparation of reference materials of total thyroxine

VernacularTitle:混合人血清中总甲状腺素标准物质的研制
Author: Weiyan ZHOU ; Songlin YU ; Donghuan WANG ; Jing WANG ; Jie ZENG ; Tianjiao ZHANG ; Jiangtao ZHANG ; Feng ZHAO ; Nan QIN ; Qinghui MENG ; Mo WANG ; Wenxiang CHEN ; Chuanbao ZHANG
Publication Type:Journal Article
Keywords: Thyroxine; Chromatograhy,liquid; Tandem mass spectrometry; Reference standards
From: Chinese Journal of Laboratory Medicine 2016;(1):40-44
CountryChina
Language:Chinese
Abstract: Objective To prepare the serum reference materials for total thyroxine .Methods Individual blood samples were collected from 13 healthy donors (7 males and 6 females) aged from 20 to 50 years old, and the sera were separated and mixed into 4 serum pools according to the concentration of thyroxine.The materials were tested for homogeneity and stability using routine methods .The method of isotope dilution liquid chromatography tandem mass spectrometry ( ID-LC/MS/MS) was used to determine the concentration of thyroxine .The candidate reference materials were also measured by four conventional methods to analyze the commutability of the materials .Results It showed that the four candidate reference materials were homogeneous and commutable in four conventional methods and they were tested to be stable for at least 1 year at -70 ℃using the isochronous stability study .The certified values ( reference value ± expanded uncertainty ,nmol/L) were:75.9 ±1.8,105.3 ±2.2,114.7 ±2.1 and 187.4 ±2.9.Conclusions Certified reference materials for serum thyroxine have been prepared .These materials have been approved to be the Certificate Reference Materials of GBW 09127,GBW 09128,GBW 09129 and GBW 09130.