Determination of 2-Indole Ketone Derivative and Its Related Substances by High Performance Liquid Chromatography
10.3870/j.issn.1004-0781.2016.04.015
- VernacularTitle:高效液相色谱法测定2-吲哚酮类衍生物含量及其有关物质?
- Author:
Yiming LI
;
Bingkun XIAO
;
Jianyun YANG
;
Rongqing HUANG
- Publication Type:Journal Article
- Keywords:
2-Indole ketone derivative;
Content determination;
Chromatography,high performance liquid
- From:
Herald of Medicine
2016;35(4):386-389
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an high performance liquid chromatography method for determination of 2-indole ketone derivative and its related substances. Methods Agilent Eclipse XDB-C18(250 mm×4.6 mm,5 μm) column was adopted.The mobile phase was acetonitrile-water with gradient elution mode at a flow rate of 1. 0 mL?min-1; the column temperature was 35 ℃;the injection volume was 20 μL and the detection wavelength was set at 257 nm. Results 2-indole ketone derivative ID and related substances could be well separated. The 2-indole ketone derivative had good linear correlation ( r=0.999 4) within the range of 40-300 μg?mL-1 . It had a good precision ( RSD<1%) . The limit of detection was 8 ng. Conclusion The method is accurate,simple,sensitive and selective,which can be used for the quality control of 2-indole ketone derivative and related substances.
