Technology of Diosgenin from Doscorea zingiberensis by Bio-enzyme Pretreatment-alcohol Extraction and Acid Hydrolysis Method
10.3870/j.issn.1004-0781.2016.04.016
- VernacularTitle:生物酶预处理-醇提酸解法提取盾叶薯蓣中薯蓣皂苷元的工艺?
- Author:
Pan LEI
;
Guangyi YANG
;
Shiming DU
;
Fang YE
;
Xiaona HUI
;
Jinbao WEI
;
Chenning ZHANG
- Publication Type:Journal Article
- Keywords:
Doscorea zingiberensis;
Diosgenin;
Enzyme;
Extractive technology
- From:
Herald of Medicine
2016;35(4):389-393
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore a clean and efficient new method for extraction of Diosgenin. Methods Yield of the total saponins was evaluated to determine the optimal enzymolysis temperature,pH,solid to liquid ratio,dosage of enzyme and enzymolysis time.Using diosgenin yield as an index,solid to liquid ratio,concentration of sulfuric acid and hydrolysis time were optimized in the saponins hydrolysis process via orthogonal experiment. Results The best conditions for the enzyme pretreatment were as follows:the temperature for enzymolysis was 70℃,pH 5.5,solid to liquid ratio was 1:4,dosage of enzyme was 8 mL?kg-1,and extraction time was 24 h.The best conditions of total saponins hydrolysis were as follows:the solid to liquid ratio was 1:4,concentration of sulfuric acid was 2.0 mol?L-1 ,and hydrolysis time was 5 h. Conclusion The new method is environmental friendly and highly efficient,and expected to be applied in industrial production.
