Extraction Technology of Flavone Ingredients of Herba Epimedii by Multi-index Uniform Design
10.3870/j.issn.1004-0781.2016.04.017
- VernacularTitle:淫羊藿黄酮类成分的多指标均匀设计提取工艺?
- Author:
Xiaojing NIU
;
Yingjie CAO
;
Jing LU
;
Xiaoying DUAN
;
Liran XU
- Publication Type:Journal Article
- Keywords:
Herba Epimedii;
Flavone ingredients;
Multiple index;
Uniform design;
Extraction technology
- From:
Herald of Medicine
2016;35(4):393-396
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize extraction technology of flavonesingredients from Herba Epimedii by uniform design. Methods Ultraviolet spectrophotometry was adopted to determine the content of total flavonoids. Content of epimedin A, epimedin B,epimedin C,icariin and baohuosideⅠ were determined by HPLC. U10?( 108 ) uniform design was used to conduct the multiple comprehensive evaluation of six ingredients and comprehensively analyze the influence of the concentration and amount of ethanol, extracting time on extraction of flavonesin gredients from Herba Epimedii. Results The uniform design experiment showed that 15-fold weight of 60% ethanol,extracting 2 times and each time with 165 min were the optimum extraction condition. Conclusion The method is easy and reasonable to handle,has stable and reliable results,and good repeatability and feasibility.It can be applied in industrial production.
