Relationship between MDSC level and immune function and HBV-DNA replication level in patients with hepatitis B
10.3760/cma.j.issn.1008-6706.2016.08.011
- VernacularTitle:骨源性抑制细胞含量与乙肝患者免疫功能及 HBV－DNA 复制水平的关系
- Author:
Xiaoyan LI
- Publication Type:Journal Article
- Keywords:
Hepatitis B;
Myeloid-derived suppressor cell;
Hepatitis B virus DNA;
Immune func-tion;
Correlation
- From:Chinese Journal of Primary Medicine and Pharmacy
2016;23(8):1161-1164
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between myeloid-derived suppressor cell ( MDSC) levels and immune function and hepatitis B virus DNA ( HBV-DNA) replication level in patients with hepatitis B. Methods 96 patients with hepatitis B were included in the study.40 normal persons who received physical examina-tion in the hospital during the same period were included as the control group.The content of MDSC was detected by flow cytometry and HBV-DNA level was detected by real-time fluorescence quantitative PCR technique.Statistical analysis was performed.Results The content of MDCS in hepatitis B group (8.77 ±3.69)%was significantly high-er than (1.64 ±1.26)%in the control group (t=16.80,P<0.05).The content of MDCS in HBsAg positive group (11.29 ±3.39)%was significantly higher than (4.71 ±2.88)%in HBsAg negative group (t=10.76,P<0.05). The proportions of HBA DNA<103 IU/mL,103 -104 IU/mL and 105 -106 IU/mL in HBsAg positive group(0.00%, 0.00%,9.09%) were significantly lower than those in HBsAg negative group (54.05%,22.97%,20.27%),while the proportion of HBA DNA>107 IU/mL ( 90.91%) was significantly higher than that in HBsAg negative group (2.70%)(P<0.05).The content of MDSC in HBV DNA positive group[(10.82 ±3.26)%] was significantly higher than that in HBV DNA negative group[(5.84 ±2.16)%](t=8.42,P<0.05).Spearman's correlation analy-sis showed that the level of MDCS expression was positively correlated with the level of DNA replication (r=0.769, P<0.05).Conclusion The increased level of MDSC content can cause the immune suppression and is positively correlated with the degree of HBV DNA replication.
