Correlationship between methylation status of cholangiocarcinoma-related genes in bile and cholan-giocarcinoma
10.3760/cma.j.issn.1007-5232.2016.02.007
- VernacularTitle:胆汁中胆管癌相关基因甲基化状态与胆管癌病变关系研究
- Author:
Lu XIE
;
Jianfeng YANG
;
Xiaofeng ZHANG
- Publication Type:Journal Article
- Keywords:
DNA methylation;
Polymerase chain reaction;
Bile duct neoplasms;
Diagnosis;
Genes,TFPI-2;
Genes,FOXE1;
Genes,NPTX2
- From:
Chinese Journal of Digestive Endoscopy
2016;33(2):93-96
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the aberrant methylation status of TFPI-2,FOXE1 and NPTX2 gene promoter region in bile from cholangiocarcinoma patients and the diagnostic value for cholangiocarcinoma. Methods Methylation—specific PCR(MSP)was used to detect the promoter methylation status of TFPI-2, FOXE1 and NPTX2 genes in bile from 29 patients with cholangiocarcinoma and 27 patients with choledocholi-thiasis.The sensitivity and specificity of combined methylation of 3 promotors and brush cytology were analyzed. Results There was significant difference between the methylation rate of NPTX2,FOXE1 in choledocholithiasis group and cholangiocarcinoma group(75. 86% VS 25. 93%,χ2 = 13. 964,P<0. 05;44. 82%VS 14. 81%,χ2 = 4. 622,P<0. 05).There was no significant difference between the methylation rate of TFPI-2 in choledocholithiasis group and cholangiocarcinoma group(17. 24%VS 18. 52%,χ2 = 0. 016,P>0. 05).Positive rate was 34. 48% with endoscopic retrograde brush cytolog,and 86. 20% with promoter methylation of FOXE1 combined with NPTX2 genes,and there was significance difference in the positive rates between the two methods(χ2 = 14. 122,P< 0. 05). Conclusion The methylation rates of NPTX2,FOXE1 were significantly higher in choledocholithiasis group than those in the cholangiocarcinoma group. Detection of comethylation rate of the FOXE1 and NPTX2 genes could improve diagnostic sensitivity of cholangiocarcinoma.
