Influence of FOXC2 in angiogenesis of breast cancer MCF-7 cells by DLL4/Notch1 signal pathway
10.13481/j.1671-587x.20140305
- VernacularTitle:FOXC2调控DLL4／Notch1信号通路对乳腺癌MCF-7细胞血管生成的影响
- Author:
Hong LIU
;
Jia XIE
;
Hao LIU
;
Yueyong ZHENG
;
Chengyi WU
;
Hongbo QU
;
Cong LI
- Publication Type:Journal Article
- Keywords:
breast neoplasms;
MCF-7 cells;
forkhead box C-2;
Notch signaling pathway;
angiogenesis
- From:
Journal of Jilin University(Medicine Edition)
2014;(3):488-492
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the influence of tranSCription factor FOXC2 in angiogenesis of breast cancer MCF-7 cells and to clarify the action mechanism of FOXC2 in promoting tumor angiogenesis.Methods FOXC2 gene and empty vector gene were transfected into breast cancer of MCF-7 cell line with FOXC2 lentivirus gene transfection technique to obtain stable transfection cell line. The MCF-7 cells were devided into non-transfected group,empty-vector group and over-expression group.Matrigel assay and Transwell chamber test were used to observe the changes of tube formation and migration ability of human umbilical vein endothelial cells (HUVECs)in MCF-7 cells supernatant in various groups. PT-PCR and Western blotting methods were applied to detect the expressions of FOXC2,DLL4 and Notch1 mRNA and protein.Results Compared with non-tranfected group and empty-vector group,the tube formation and the migration number of HUVECs in FOXC2 over-expression group were increased(P<0.05);the expressions of FOXC2,DLL4 and Notch1 mRNA and proteins were significantly increased(P<0.05).Conclusion The FOXC2 over-expression in MCF-7 cells can increase the tube formation ability and migration ability of HUVECs,and its mechanism may be related to Notch signaling pathway.
