Effects of puerarin on interstitial cells of Caj al and expression of c-kit protein in colon tissue of mice with chronic alcoholism
10.13481/j.1671-587x.20140325
- VernacularTitle:葛根素对慢性酒精中毒小鼠结肠组织Caj al间质细胞和C-kit蛋白表达的影响
- Author:
Baijuan XIA
;
Shuqin CUI
- Publication Type:Journal Article
- Keywords:
puerarin;
chronic alcoholism;
interstitial cells of Cajal;
C-kit protein;
ultrastructure;
colon
- From:
Journal of Jilin University(Medicine Edition)
2014;(3):588-592
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the protective effects of puerarin on the colon tissue of the mice with chronic alcoholism through observing the changes of quantity and morphology of interstitial cells of Cajal (ICC)and the expression of c-kit protein in colon tissue of chronic alcoholism mice.Methods 24 healthy BALB/C mice were randomly divided into saline control group (SC group ), chronic alcoholic intoxication group (CAI group ) and puerarin pretreatment group(PUE group).The models of chronic alcoholism mice were established in CAI group and PUE group. The intestinal transmission rate was tested by measuring the Indian ink advancing length;immunofluorescence and transmission electron microscope (TEM)were applied to detect the distribution, quantity and ultrastructure of ICC;Western blotting method was used to analyze the C-kit protein expression.Results The intestinal transmission rate in PUE group was significant higher than that in CAI group(P<0.05),but was still lower than that in SC group (P<0.05);compared with CAI group,the number of ICC in PUE group was increased (P<0.05),but there was no significant difference between SC group and PUE group(P>0.05).Compared with SC group,the ultrastructure of ICC in CAI group had obvious changes,the organelles were decreased,and the mitochondrion was swelling.But those changes were reversed in PUE group and the number of mitochondria was increased.The expression of C-kit protein in PUE tissue was higher than that in CAI group (P<0.05),but there was no significant difference compared with SC group.Conclusion Puerarin has a repair or reverse effect on the changes of the number,ultrastructure of ICC and C-kit protein expression caused by alcohol.