The Comparison of Duration to Maintain Cell Viability for HLA-B27 Test According to Anticoagulants.
- Author:
Hong Seok KIM
1
;
La He JEARN
;
Think You KIM
Author Information
1. Department of Diagnostic Immunology/Laboratory Medicine, The Hospital for Rheumatic Diseases, Hanyang University Medical Center, Seoul, Korea. tykim@hanyang.ac.kr
- Publication Type:Original Article
- Keywords:
HLA-B27;
Cell Viability;
Heparin;
ACD
- MeSH:
Anticoagulants*;
Cell Survival*;
Heparin;
HLA-B27 Antigen*;
Lymphocytes;
Refrigeration
- From:The Journal of the Korean Rheumatism Association
2004;11(3):247-253
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: HLA-B27 test require at least 80% of viable cells. So the procedures require immediate processing of the blood samples. To increase the efficiency and the cost-effectiveness of the laboratory, longer maintenance of cell viability is requested. For this purpose, we compared the cell viability of blood samples collected in heparin tubes with those collected in ACD tubes. Our aim was to determine whether the length of storage that could maintain adequate cell viability for HLA-B27 testing depends on the anticoagulants in the storage tube and to ascertain the qualified HLA-B27 results after several days of storage. METHODS: We collected 15 blood samples in ACD and heparin tubes. The samples were stored at 4oC for 6 days and after which, 2 mL of the sample was used to confirm the viability of lymphocytes every 24 hours for 5 days. For evaluating the reproducibility, the HLA-B27 test was performed at the day of sampling and after 6 days of refrigeration. RESULTS: There was no difference in cell viability between ACD and heparin until the sixth day, but a statistically difference was observed from the seventh day (p=0.004). The HLA-B27 test showed no different results even after 6 days of storage. CONCLUSION: We suggest that the heparin tubes have a same storage benefit as the ACD tubes of blood for HLA-B27 testing for less than a week. This can affect more economic and efficient laboratory management for HLA-B27 testing.
