Effect of continuous renal replacement on Treg/Th17 and related inflammatory factors in patients with severe sepsis
10.3760/cma.j.issn.1008-6706.2016.07.004
- VernacularTitle:连续性肾脏替代治疗对严重脓毒症患者Treg／Th17及其相关炎性因子的影响
- Author:
Lu LU
;
Linxia WANG
;
Luming TANG
;
Chunxue YAN
;
Guoquan PAN
- Publication Type:Journal Article
- Keywords:
Sepsis;
Renal replacement therapy;
T-lymphocytes
- From:
Chinese Journal of Primary Medicine and Pharmacy
2016;23(7):973-977
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of continuous renal replacement therapy ( CRRT) on the reg-ulation of Treg/Th17 in patients with severe sepsis,and related inflammatory factors IL-6,IL-17,IL-10,and TNF-α.Methods 60 patients with severe sepsis were randomly divided into two groups,30 cases in each group.The control group received conventional treatment,and the observation group was treated with CRRT on the basis of the control group.Flow cytometry was used to detect the levels of Tregs and Th17 cells,and IL-6,IL-10,IL-17 and TNF-αwere detected by ELISA method.At the same time,the APACHEII score,ICU length of hospital stay were observed and recorded.Results After treatment,APACHE Ⅱscore,ICU length of hospital of the observation group were lower than the control group,there were statistically significant differences(t=4.258,t=4.518,all P<0.05). The expression levels of Th17,Treg and Tregs/Th17 in the observation group were significantly lower than the control group,the differences were statistically significant (t=5.872,4.267,4.285,all P<0.05).The expression levels of IL-6,IL-17,TNF-αin the observation group decreased more significantly than the control group,the differences were statistically significant (t=5.829,5.257,5.983,all P<0.05).But the expression level of IL-10 in the two groups had no obvious change[(35.26 ±18.63) pg/mL vs (35.52 ±20.09) pg/mL,t=2.164,P>0.05].Conclusion CRRT can not only remove the inflammatory mediators of abnormal expression,improve the function of T cells,but also can maintain the balance between Th17 and Treg,improve the immune disorders,and improve the prognosis of sepsis.
