Comparison of ion selectivity electrode assay and arsenazo Ⅲ assay in determination serum calcium concen-tration

VernacularTitle:离子选择电极法与偶氮胂Ⅲ法检测血钙的比较研究
Author: Shengya CAO ; Jia LI ; Lei LI ; Wei YIN ; Hongpeng YANG
Publication Type:Journal Article
Keywords: Calcium; Laboratory techniques and methods
From: Chinese Journal of Primary Medicine and Pharmacy 2016;23(5):737-739
CountryChina
Language:Chinese
Abstract: Objective To evaluate the correlation of the ion selectivity electrode assay and arsenazo Ⅲ assay for serum calcium determination and compare the differences between two methods.Methods 142 serum samples were collected from patients and detected by ion selectivity electrode assay and arsenazo Ⅲ assay,the differences of two methods were compared,person correlation and Bland -Altman was used to discuss the correlation.Results The serum calcium concentration detected by ion selectivity electrode assay was (2.23 ±0.10)mmol/L and detected by arsenazo Ⅲ assay was (2.24 ±0.12)mmol/L,the difference between the two methods was not statistically significant (t =-1.09,P >0.05).The person correlation analysis demonstrated that the two methods had a positive correlation (r =0.393,P <0.01).The Bland -Altman consistency analysis showed that ion selectivity electrode assay was agreed with arsenazo Ⅲ assay.Conclusion The two methods for serum calcium concentration determination,the ion selectivity electrode assay was coincidence with the arsenazo Ⅲ assay.