Expression and significance of miRNA-34a in cervical intraepithelial neoplasia
10.3760/cma.j.issn.1008-6706.2016.05.035
- VernacularTitle:宫颈上皮内瘤变组织和血清中微小 RNA－34a 的表达及临床意义
- Author:
Hui YUAN
;
Shoufang KONG
;
Jinyan ZHONG
;
Xuan SUN
- Publication Type:Journal Article
- Keywords:
miRNA -34a;
Cervical intraepithelial neoplasia;
Polymerase chain reaction
- From:
Chinese Journal of Primary Medicine and Pharmacy
2016;23(5):768-770
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression of miRNA -34a in normal cervical,low grade cervical intra-epithelial neoplasia and high grade cervical intraepithelial neoplasia,to explore its clinical significance.Methods The expression levels of miRNA -34a in 82 cases of low grade cervical intraepithelial neoplasia (CINⅠ,CIN L group),87 cases of high grade cervical intraepithelial neoplasia (CINⅡ -Ⅲ,CIN H group) and 60 cases of normal cervical tissues (control group) in tissue specimens and serum were examined by real -time PCR(RT -PCR). Results The relative expression levels of miRNA -34a in tissues and serum of CIN H group were (0.584 ± 0.288),(0.528 ±0.299),which of CIN L group were (0.903 ±0.252),(0.847 ±0.279),which of the control group were (0.960 ±0.212),(0.908 ±0.223).The miRNA -34a level of CIN H group in tissues and serum were lower than CIN L group and control group,there was significant difference( Ptissues =0.001,Pserum =0.003).The miRNA -34a level in tissue and serum had no significant difference between the CINL group and control group(P >0.05).The expression level of serum miRNA -34a was positively related with the level of the tissues(r =0.907,P <0.01).Conclusion The expression level of miRNA -34a in high grade CIN was significantly decreased,which indi-cated that miRNA -34a play an important role in the prognosis of CIN and the transformation of cervical cancer,and it has certain clinical value for the diagnosis and treatment of CIN.
